Preliminary studies on the titration and neutralization test of Japanese B encephalitis virus with plaque assay method
Chen, B.C.; Hsu, C.H.; Liu, Y.Y.; Fan, R.L.
Acta Microbiol Sinica 9(1): 53-58
Infectivity titers of Japanese B encephalitis virus obtained with the plaque assay method were approximately lower by 1 log than those obtained through intracerebral inoculation of mice. However, the ratio between these 2 sets of titers was relatively constant and the number of plaques formed was proportional to the quantity of virus inoculated. Therefore it is suggested that the plaque assay method may be used for routine quantitative titrations of the infectivity of Japanese B encephalitis virus. By comparing the results obtained in the neutralization tests, it was found that the neutralization index of a given serum obtained with the plaque assay method was 2[long dash]6 times higher than that obtained with the mouse brain inoculation method. Certain factors which were considered to be essential for the titration and neutralization test of Japanese B encephalitis virus with the plaque assay method are discussed.