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Chapter 25,315

Prufung der Wider-standsfahigkeit des Virus der Bornaschen Krankheit und der Hiihnerpocken gegen chemische Desinfektionsmittel, insbesondere gegen Trosilin

Witte, J.; Schaal, J.

Zeitschr Infektionskr U Hyg Haustiere 45(4): 265-272

1934


Accession: 025314206

Borna Disease: The virus was suspended in equal amounts of saline and absorbed by a 2 X 2 cm. piece of blotting paper for 1/2 hr., and then exposed to the disinfecting sol., and the disinfectant neutralized. After grinding the paper in saline, 0.25 cc of the extract was injected intracerebrally into rabbits. The virus was not destroyed by soda lye (1% sol.) in 30 min., while lime water (1:20), formalin (Wo), and HgCl2 (1:1000) failed in 10 min., and a 3% soda sol. was without effect in 2 hrs. 3% phenol killed the virus in 10 min., as did 0.5% trosilin. A flotation method, in which a 1:20 emulsion of infected brain tissue was mixed with disinfectant, gave more satisfactory results. Soda sol. was not effective in 2 hrs., but 0.5% sol. of trosilin was effective in 5 min., 1% soda lye sol. in 30 min.; and 1:20 lime water, 1% formalin and 1:1000 HgCl2 sol. destroyed the virus in 10 min.[long dash]Fowl pox: Since fowl-pox virus is highly resistant to external conditions, a satisfactory germicidal agent is especially desirable. Various methods were tested on the virus used, but inoculation into the upper shank of a susceptible chicken was particularly valuable as a gauge of virulence. 2% soda lye was not effectively virucidal in 60 min.; 1% trosilin and 3% phenol were effective in 5 min.

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