Section 26
Chapter 25,327

Quantification of water buffalo (Bubalus bubalis) cytokine expression in response to inactivated foot-and-mouth disease (FMD) vaccine

Mingala, C.N.; Konnai, S.; Venturina, F.A.; Onuma, M.; Ohashi, K.

Research in Veterinary Science 87(2): 213-217


ISSN/ISBN: 1532-2661
PMID: 19303121
DOI: 10.1016/j.rvsc.2009.02.008
Accession: 025326847

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This study describes the quantification of cytokine expression of vaccinated water buffaloes with FMD inactivated vaccine. Using real-time PCR quantification assay, expression of Th1 (IL-2, IL-12p40, IFNgamma); Th2 (IL-4, IL-10) and inflammatory (IL-6, TNFalpha) cytokines were quantified weekly for the entire three-week duration of the experiment. It was noted that IFNgamma, IL-10 and TNFalpha had peaked on week three post-vaccination while the remaining cytokines peaked on the second week and decreased by the third week. The counteraction between IFNgamma and IL-4 was noted as well as the possible suppressive action of IL-10 to that of IL-2 and IL-12, which is a common phenomenon between Th1 and Th2 cytokines. Synergy between TNFa and IL-6 was also observed. These findings suggest that within the immune system of water buffalo there is a dynamic cell-mediated and humoral interaction in response to immunogen. This assessment of the cytokine expressions is vital for the study of water buffalo disease progression and concurring protective immune responses.

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