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Quantitative separation and determination of bilirubin and conjugated bilirubin in human serum

Weber, A.P.; Schalm, L.

Clinica Chimica Acta; International Journal of Clinical Chemistry 7: 805-810

1962


ISSN/ISBN: 0009-8981
PMID: 13999362
DOI: 10.1016/0009-8981(62)90063-3
Accession: 025330513

A simple method - suitable for use in the clinical chemical laboratory - is described for the determination of bilirubin and conjugated bilirubin in human serum, the pigments being separated before the quantitative determination. The repro-ducibility of the method is about 0.10 mg of pigment per 100 ml of serum. The presence of conjugated bilirubin can be visually established from about 0.15 mg/100 ml upwards. No interference is caused by serum turbidity, or by moderately hemolytic sera. Deviating azo colors, such as those which occasionally occur in other determinations, were not observed. The method can be used for determinations in small quantities of serum (e.g. 0.04 ml), so that neonatal blood can also be examined.

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