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Rationalization of methods used for determining blood sugar by the Hagedorn and Jensen method

Rationalization of methods used for determining blood sugar by the Hagedorn and Jensen method

Lab Delo 8(11): 57-58

The author describes measures taken to shorten the time required for the analysis of blood sugar. Samples were filtered through cotton which had first been washed with water. A 100 ml rubber bulb was used to force water out of the cotton. A 60-65 ml home-made pipette or a 100 ml burette was used to rinse out tubes in which blood had been placed together with hydrated ZnO. Instead of using a mixture of ZnSO4, NaCl, and KI, which must be prepared no more than 24 hrs. before use, this mixture was combined with acetic acid and dispensed in 4 ml portions from a 50 ml burette. Addition of acetic acid was found to prevent liberation of free iodine so that the mixture could be stored for as long as 10 days. Use of these measures to determine sugar in 2 parallel tests reduces the time required from an average of 15 minutes to 5 minutes.

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