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Resolution of complex mixtures of nucleic acid bases, nucleosides, and nucleotides by two-dimensional thin-layer chromatography on polyethyleneimine-cellulose



Resolution of complex mixtures of nucleic acid bases, nucleosides, and nucleotides by two-dimensional thin-layer chromatography on polyethyleneimine-cellulose



J Chromatogr 35(4): 531-537



A method is given for resolving a complex mixture of nucleic acid bases, nucleosides and nucleotides on polyethyleneimine-cellulose MN-300 chromatofilms. The nucleotides are prevented from migrating when the individual nucleic acid bases and nucleosides are separated from each other. Also, in the couse of the development that resolves the nucleotides, the nucleic acid bases and nucleosides disappear. Thus, the procedure simplifies the isolation and detection of individual components of a complex mixture of nucleic acid derivatives. The method was applied satisfactorily to the analysis of alkali hydrolysates of the transfer RNAs of Escherichia coli B and of yeast.

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Accession: 025391233

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DOI: 10.1016/s0021-9673(01)82420-1


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