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Sensitive isothermal detection of nucleic-acid sequence by primer generation-rolling circle amplification

Sensitive isothermal detection of nucleic-acid sequence by primer generation-rolling circle amplification

Nucleic Acids Research 37(3): E19-E19

A simple isothermal nucleic-acid amplification reaction, primer generation-rolling circle amplification (PG-RCA), was developed to detect specific nucleic-acid sequences of sample DNA. This amplification method is achievable at a constant temperature (e.g. 60 degrees C) simply by mixing circular single-stranded DNA probe, DNA polymerase and nicking enzyme.

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Accession: 025437212

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PMID: 19106144

DOI: 10.1093/nar/gkn1014

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