+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Some Notes On The Starch-Gel Electrophoresis Of Hemoglobins

Some Notes On The Starch-Gel Electrophoresis Of Hemoglobins

Journal of Laboratory and Clinical Medicine 63: 290-296

Starch-gel electrophoresis of hemoglobin solutions was carried out in 2 different buffer systems of approximately the same pH. The tris-citrate buffer was noted to provide sharp definition of the more slowly moving hemoglobins at pH 8.7 but was inadequate for the separation of Hgb F from Hgb A. Methemoglobin forms of various hemoglobin types moved more slowly than the oxy, reduced, CO, or cyanmethemoglobin compounds. Electrophoresis in a lithium buffer system, pH 8.04, provided sharp definitions of hemoglobins with a mobility equal to or greater than that of Hgb S, permitted a sharp distinction of Hgb F from Hgb A and demonstrated an even more striking displacement cathodally of the methemoglobin forms of the various hemoglobin types.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 025489346

Download citation: RISBibTeXText

PMID: 14125115

Related references

Studies of Abnormal Hemoglobins. I. Procedure for Quantitation of Hemoglobins Separated by Means of Starch Gel Electrophoresis. American Journal of Clinical Pathology 40: 227-238, 1963

Separation of Human Hemoglobins with Starch Gel Electrophoresis, Aluminum Oxide Chromatography and Deae Cellulose Chromatography. I. Technics and Results of the Study of Adult and Fetal Hemoglobins. Acta Medica Iugoslavica 17: 252-262, 1963

Starch gel electrophoresis of hemoglobins. Rev Francaise Etud Clin Et Biol 3(4): 377-380, 1958

Starch Gel Electrophoresis Of Fish Hemoglobins-Ii. Nippon Suisan Gakkaishi 31(10): 833-839, 1965

Starch Gel Electrophoresis Of Fish Hemoglobins-I. Nippon Suisan Gakkaishi 31(10): 827-832, 1965

Starch-gel Electrophoresis of Fish Hemoglobins-III. Nippon Suisan Gakkaishi 33(3): 195-203, 1967

Study of mouse hemoglobins by starch-gel electrophoresis. FED PROC 22(2 Pt 1): 601, 1963

Quantitation of hemoglobins A and S separated by starch gel electrophoresis. Analytical Biochemistry 22(1): 21-26, 1968

Separation of human hemoglobins by starch-gel zone electrophoresis. Clinica Chimica Acta; International Journal of Clinical Chemistry 6: 136-141, 1961

Quantitative starch-gel zone electrophoresis of human hemoglobins. Acta Pharmaceut Jugoslav 13(2): 69-76, 1963

Use of a non-continuous buffer system for starch gel electrophoresis of hemoglobins. Revue Francaise d'Etudes Cliniques et Biologiques 3(8): 877-880, 1958

Thin-layer starch gel electrophoresis of various hemoglobins and nonhemoglobin erythrocytic proteins (NHP). Igaku to Seibutsugaku. Medicine and Biology 75(5): 180-185, 1967

Starch-gel Electrophoresis of Hemoglobins of Freshwater Salmonid Fishes in Northeast Japan. Nippon Suisan Gakkaishi 39(4): 449-459, 1973

Starch-gel electrophoresis of hemoglobins of freshwater salmonid fishes in northwest Japan. Bulletin Jap Soc Scient Fish 394: 449-459, 1973

Starch-gel Electrophoresis of Hemoglobins of Freshwater Salmonid Fishes in Southwest Japan-II. Nippon Suisan Gakkaishi 39(1): 97-114, 1973