+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Southern rice black-streaked dwarf virus A new proposed Fijivirus species in the family Reoviridae

Southern rice black-streaked dwarf virus A new proposed Fijivirus species in the family Reoviridae

Chinese Science Bulletin 53(23): 3677-3685

For the past several years, a novel dwarf disease has been observed on rice (Oryza sativa) in some regions of Guangdong Province and Hainan Province, southern China. Infected plants showed stunting, dark leaf and small enations on stem and leaf back. Typical Fijivirus viroplasma containing crystalline arrayed spherical virons approximately 7075 nm in diameter and tubular structures were detected in ultrathin sections by an electron microscope in parenchyma phloem cells of the infected plants. The virus was transmitted to rice seedlings by white-backed planthoppers, Sogatella furcifera (Hemiptera: Delphacidae), collected in the diseased fields. Analysis of dsRNA extracts from infected plants revealed ten linear segments, which were similar to the electrophoretic profile of Rice black-streaked dwarf virus (RBSDV). RT-PCR with a single primer which matched to a linker sequence ligated to both 3 ends of the viral genomic dsRNAs resulted in amplification of genome segments 9 (S9) and 10 (S10) cDNA products. The complete nucleotide sequences of S9 and S10 were obtained from clones of the RT-PCR amplicon exhibited characteristic properties of Fijivirus including low GC content (34.5% and 35.6%), genus conserved 5 and 3 termini sequences and similar genome organization. Blast searches indicated that the sequences of S9 and S10 shared 68.8%74.9% and 67.1%77.4% nucleotide identities with those of viruses in the Fijivirus group 2, respectively. These values were similar to those among other viruses in the Fijivirus group 2 and considerably lower than those among RBSDV isolates. Phylogenetic trees based on S9 and S10 nucleotide sequences and their putative amino acid sequences showed that this virus represented a separate branch among other Fijiviruses. The virus was also detected by a nested RT-PCR assay in corn (Zea mays), barnyard grass (Echinochloa crusgalli), Juncellus serotinus and flaccidgrass (Pennisetum flaccidum) in and/or adjacent to the infected rice fields. It is proposed that this virus be considered as a new species, Southern rice black-streaked dwarf virus, in the group 2 of the genus Fijivirus in the family Reoviridae.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 025502427

Download citation: RISBibTeXText

DOI: 10.1007/s11434-008-0467-2

Related references

Biology of Southern rice black-streaked dwarf virus: a novel fijivirus emerging in East Asia. Plant Pathology 66(4): 515-521, 2017

Southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in Asia. Frontiers in Microbiology 4: 270, 2013

The Complete Genome Sequence of Two Isolates of Southern rice black-streaked dwarf virus, a New Member of the Genus Fijivirus. Journal of Phytopathology 158(11-12): 733-737, 2010

Characterization of homologous and heterologous interactions between viroplasm proteins P6 and P9-1 of the fijivirus southern rice black-streaked dwarf virus. Archives of Virology 160(2): 453-457, 2015

The P7-1 protein of southern rice black-streaked dwarf virus, a fijivirus, induces the formation of tubular structures in insect cells. Archives of Virology 156(10): 1729-1736, 2011

Simultaneous detection and differentiation of Rice black streaked dwarf virus (RBSDV) and Southern rice black streaked dwarf virus (SRBSDV) by duplex real time RT-PCR. Virology Journal 10: 24, 2013

Close evolutionary relationship between rice black-streaked dwarf virus and southern rice black-streaked dwarf virus based on analysis of their bicistronic RNAs. Virology Journal 16(1): 53, 2019

Quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time. Virus Research 208: 146-155, 2015

Interactions between the P6 and P5-1 proteins of southern rice black-streaked dwarf fijivirus in yeast and plant cells. Archives of Virology 158(8): 1649-1659, 2013

Differences in Molecular Characteristics of Segment 8 in Rice black-streaked dwarf virus and Southern rice black-streaked dwarf virus. Plant Disease 102(6): 1115-1123, 2018

Identification of rice black-streaked dwarf fijivirus in maize with rough dwarf disease in China. Archives of Virology 146(1): 167-170, 2001

NAC transcription factor family genes are differentially expressed in rice during infections with Rice dwarf virus, Rice black-streaked dwarf virus, Rice grassy stunt virus, Rice ragged stunt virus, and Rice transitory yellowing virus. Frontiers in Plant Science 6: 676, 2015

Occurrence of rice black-streaked dwarf Fijivirus in Hokkaido. Annual Report of the Society of Plant Protection of North Japan ( 46): 38-41, 1995

A black-streaked dwarf disease on rice in China is caused by a novel fijivirus. Archives of Virology 153(10): 1893-1898, 2008

Detection and assignment of proteins encoded by rice black streaked dwarf fijivirus S7, S8, S9 and S10. Journal of General Virology 79: 1487-1494, 1998