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Spread of a large plasmid carrying the cpe gene and the tcp locus amongst Clostridium perfringens isolates from nosocomial outbreaks and sporadic cases of gastroenteritis in a geriatric hospital

Kobayashi, S.; Wada, A.; Shibasaki, S.; Annaka, M.; Higuchi, H.; Adachi, K.; Mori, N.; Ishikawa, T.; Masuda, Y.; Watanabe, H.; Yamamoto, N.; Yamaoka, S.; Inamatsu, T.

Epidemiology and Infection 137(1): 108-113

2008

ISSN/ISBN: 0950-2688
PMID: 18485266
DOI: 10.1017/s0950268808000794
Accession: 025514753
SUMMARYTo investigate two clusters of diarrhoea cases observed in our geriatric hospital wards, the faecal specimens were analysed. Reversed passive latex agglutination assay revealed that 63.2% and 41.7% of the faecal specimens from each cluster were positive for Clostridium perfringens enterotoxin. PCR assay revealed that 71.4% and 68.8% of C. perfringens isolates from each cluster were positive for the enterotoxin gene (cpe).

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Related References

S.Kobayashi, A.W.,S.S.,M.A.,H.H.,K.A.,N.M.,T.I.,Y.M.,H.W.,N.Y.,S. 2009: Spread of a Large Plasmid Carrying the cpe Gene and the tcp Locus Amongst Clostridium perfringens Isolates from Nosocomial Outbreaks and Sporadic Cases of Gastroenteritis in a Geriatric Hospital Epidemiology and Infection 137(1): 108-113
Miyamoto, K.; Wen, Q.; McClane, B.A. 2004: Multiplex PCR genotyping assay that distinguishes between isolates of Clostridium perfringens type a carrying a chromosomal enterotoxin gene (cpe) locus, a plasmid cpe locus with an IS1470-like sequence, or a plasmid cpe locus with an IS1151 sequence Journal of Clinical Microbiology 42(4): 1552-1558
Tanaka, D.; Isobe, J.; Hosorogi, S.; Kimata, K.; Shimizu, M.; Katori, K.; Gyobu, Y.; Nagai, Y.; Yamagishi, T.; Karasawa, T.; Nakamura, S. 2003: An outbreak of food-borne gastroenteritis caused by Clostridium perfringens carrying the cpe gene on a plasmid Japanese Journal of Infectious Diseases 56(3): 137-139
Eisgruber, H.; Wiedmann, M.; Stolle, A. 1995: Use of plasmid profiling as a typing method for epidemiologically related Clostridium perfringens isolates from food poisoning cases and outbreaks Letters in Applied Microbiology 20(5): 290-294
Eisgruber, H. 1997: Die Plasmidmusteranalyse zur Stammdifferenzierung von Clostridium perfringens aus Lebensmittelvergiftungen - Plasmid profiling for strain differentiation of Clostridium perfringens from food poisoning cases and outbreaks Archiv für Lebensmittelhygiene 48(1): 10-13
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