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Studies on a riboflavin excreting yeast. I. Influence of some environmental factors on the growth and riboflavin production



Studies on a riboflavin excreting yeast. I. Influence of some environmental factors on the growth and riboflavin production



Jour Sci And Ind Res 11b: 109-115



The factors influencing the performance of a riboflavin-excreting mutant yeast strain, BY 2, were investigated. Biotin is the only vitamin essential for the growth of the organism and for the production of riboflavin. A synthetic medium containing glucose, 4 %; Difco yeast extract, 0.04 %; and salts gave satisfactory growth of the organism and a yield of 65.2[mu]g. of riboflavin per ml. of culture medium after 7 days' incubation. Better growth was observed when the concn. of yeast extract in the medium was increased, but the yield of riboflavin was markedly decreased. The growth and production capacity of the organism increase, up to a limit, with the increase in the surface area per unit volume of the culture medium.

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