Studies on boar seminal plasma proteins. III. Fractionation by gel filtrations, ion exchange and other means

Boursnell, J.C.; Nelson, M.; Cole, D.M.

Biochim Biophys Acta 117(1): 134-143

1966


Accession: 025555016

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Abstract
Sephadex G-200 separates the proteins of boar seminal plasma into 2 major components. The larger (80-90% of the total) has a sedimentation constant of 2.3 S and represents protein which is heat coagulable. It corresponds broadly with the proteins which are soluble at low ionic strength (I 0.015; pH 8). The smaller (10-20% of the total) has a sedimentation constant of between 7 and about 40 S and represents protein which is not precipitated by 12% Na2SO4 or by low ionic strength. It does, however, precipitate at low ionic strength on addition of an aliquot of the 2.3 S material from Sephadex G-200. The precipitate at low ionic strength produced by a mixture of 7-S with an aliquot of the 2.3-S proteins changes slowly and irreversibly into a form, the soluble fraction of which has a sedimentation constant of 5-S. The copious precipitate which appears on cooling samples of seminal plasma from some boars is also a 7-S protein.