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Studies on mixed infection with Newcastle disease virus. II. The occurrence of Newcastle disease virus heterozygotes and study of phenotypic mixing involving serotype and thermal stability



Studies on mixed infection with Newcastle disease virus. II. The occurrence of Newcastle disease virus heterozygotes and study of phenotypic mixing involving serotype and thermal stability



Virology 9: 649-670



Mutants of two strains of Newcastle disease virus that differ from each other in 3 characteristics (plaque type, heat stability, and serotype) were used for mixed infection of chick embryo cells. Of the virus, 90% of the plaques contain only one parental virus type but 10% contained both parental types. These mixed plaques were initiated by heterozygous particles that were probably diploid. A high proportion of the particles from mixed infections showed evidence of phenotypic mixing both in respect to serotype and thermal stability. When the fluid from mixed infections was used to singly infect cells, there were large numbers of phenotypically mixed particles in the yield. The amount of phenotypic mixing could be accounted for by the number of heterozygotes in the inoculum. Mixed infections at low multiplicities yielded five to ten times as much phenotypically mixed virus as could be accounted for by the number of cells expected to be mixedly infected by two plaque-forming par-ticles. The results suggest that nonplaque-forming particles may contribute to the phenotype of new virus derived by infection with plaque-forming virus.

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Accession: 025563284

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PMID: 13828940


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