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Studies On The Feulgen Reaction With Histochemical Model Systems



Studies On The Feulgen Reaction With Histochemical Model Systems



Journal of Histochemistry and Cytochemistry 12: 758-767



It is concluded that the shoulder in the pararosaniline spectrum is most probably caused by a configura-tional isomer of the dye as indicated by Lewis et al. for crystal violet. To expalin the abnormal spectrum of the stained DNA-cellulose, it is proposed that in this case pararosaniline is bound with the 3 amino groups to 3 neighboring deoxyribose groups in the hydrolyzed DNA. Studies with Stuart models of the molecules involved showed that in this case one conformation isomer of pararosaniline can be constructed more easily than the other. This situation may explain the spectrum of the stained DNA-cellulose. The other spectral results could also be interpreted on basis of this hypothesis.

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Accession: 025569546

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PMID: 14221058

DOI: 10.1177/12.10.758


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