Studies on the bacterial cell wall. V. Electron microscope studies of the cell wall of Salmonella typhi H-901 and the preliminary experiment for the morphological studies of the changes in the cell wall induced by the antigen-antibody reaction
Yoshida, Nagayuki; Fukuya, Isamu; Kakutani, Isao; Tanaka, Saburo; Takaishi, Kenji; Nishino, Kenji; Hashimoto, T.
Tokushima Jour Exptl Med 1(2): 105-114
No fine structure is visible in the intact cell wall of S. typhi H-901 even at a high magnification (70,000 x). Disc-like debris connected one after another with filamentous substances were produced by heat on the electron micrograph. Exposure to a 1/100 [image] NaOH solution resulted in the appearance of granular structure in the cell wall suggesting there are at least 2 electron microscopically different materials resistant to this treatment. When the cell wall was treated with 1 [image] HC1, it gave rise to fine filamentous substances 20-30 mu in width which disappeared after 60 minutes' treatment, minute granular substances were observed on the electron micrograph. A considerable increase in contrast was obtained when the cell wall was exposed to antiserum and stained with phosphotungstic acid. For the control normal rabbit serum was employed. Application of electron micrography for better understanding of the antigen-antibody reaction is discussed. The difference in degree of increase in contrast in the cell wall suggests the possibility of the arrangement of antigens in the mosaic mode. The role that the cell wall plays in immunochemistry is now being intensively investigated in our laboratory. The report dealing with this subject will be published in the future issue.