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Studies on the changes of serum cholinesterase activity in immunoreactions. V. On changes of cholinesterase activity in the serum of guinea pigs in sensitization and anaphylactic shock



Studies on the changes of serum cholinesterase activity in immunoreactions. V. On changes of cholinesterase activity in the serum of guinea pigs in sensitization and anaphylactic shock



Shikoku Acta Med 15(1): 108-115



Guinea pigs were injected subcutaneously with 1.0 ml of 1 to 100 dilution of horse serum. Three weeks after sensitization the animals were challenged by the intravenous injection of horse serum. Doses of horse serum ranged from 0.2 to 0.02 ml. Cholinesterase activity in the serum was determined colorimetrically by Takahashi-Shibata method with blood obtained by cardiac puncture. Sensitization did not bring about any appreciable change in the serum cholinesterase activity. There were no appreciable changes in the serum cholinesterase activity in the minutes after the provocative injections, but the activity decreased from 30 minutes to 24 hours after the provocative injections.

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