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Studies on the cytology of yeasts. III. The technique of handling yeasts for cytological investigations



Studies on the cytology of yeasts. III. The technique of handling yeasts for cytological investigations



Proceedings Nation Inst Science India 14(7): 315-323



The need for a clear distinction between (a) the handling of cultures of yeasts for cytological investigations and (b) the methods of fixation and staining employed to demonstrate the mitotic stages is emphasized. Some recent publications are critically evaluated and it is shown that the confused state of our knowledge of the cytology of yeasts is not due to lack of criteria to identify the various cell organelles. Criteria for the identification of nuclei and chromosomes are restated and the question of the specificity of the Feulgen reaction is reviewed. Details are given of the prepn. of wort and wort-agar, the methods of seeding and smearing and the methods of fixation and staining. An explanation is offered of the principles on which the technique of handling yeasts for cytological investigations was devised. If material is handled in the manner indicated, the mitotic stages can be studied in a regular sequence without the necessity of trying to fit the various stages into their proper sequence as in a jig-saw puzzle.

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