Studies on the cytoplasmic granules stainable with carcinogenic azo-dye, o-amino-azo-toluene. I. Classification of cytoplasmic granules in amphibian erythrocytes and their cytochemical natures
Nagatani, Y.
Japanese Jour Zool 12(2): 163-177
1958
Accession: 025575635
Cytoplasmic granules of amphibian erythrocytes are classified into 2 kinds of granules by using vital or supravital staining; one is "optically invisible granules" which are not detected with cytochemical methods but are observable with vital or supravital staining, and the other is "refractive granules" which are pre-existing granules. The optically invisible granules are further divided into 2 groups; one is "neutral red spheres" which appear before the nucleus is vitally stained with neutral red and have a tendency to increase in the apparent size under the staining conditions, and the other is "neutral red reticula" which are observed after the nucleus has been supravitally stained with it. The refractive granules are also further divided into 2 groups; one is "basophilic granules" which are easily stained vitally or supravitally with neutral red before the nucleus is stained with it but do not increase in the apparent size under the staining conditions as neutral red spheres do, and the other is "oil yellow granules" which are not stained vitally or supravitally with neutral red before the nucleus is stained with it but become stainable with it after the nucleus has been stained. Basophilic granules having the natures of Golgi bodies consist of 2 kinds of granules; dispersing granules and clustering granules; and oil yellow granules (OYG) having the natures of mitochondria consist of 2 kinds of granules; granular OYG and filamentous OYG. These refractive granules in amphibian erythrocytes are easily demonstrated with the OYG method using the carcinogenic azo-dye, o-amino-azo-toluene.