Studies on the development of eye-antennal discs of Drosophila melanogaster in tissue culture. L Effects of the facet-increasing substances upon the growth and differentiation of eye-antennal discs

Fujio, Y.

Japanese Jour Genetics 35(11): 361-370

1960


Accession: 025576072

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Abstract
The eye-antennal discs and the cephalic complexes taken out from the mature 3d-instar larvae of several eye-mutant strains of Drosophila melanogaster were cultured in vitro in synthetic media containing facet-increasing substances as ammonium lactate, acetamide and urea. The cultured eye-antennal discs were microscopically observed after an incubation of 48 hours. The Oregon-R strain was used as a wild type and B, B (bb)-l, B(coiso), B;ell (coiso), bar-3(coiso), ey2 (coiso) and Dp strains as the eye-mutants. The amount of the metamorphic hormone secreted from 5 cephalic complexes in a hanging-drop seems to be sufficient for the growth and differentiation of the eye-antennal discs. The cephalic complexes of several eye-mutant larvae seem to provide some unknown factors promoting the growth and differentiation which are characteristic to some eye-mutant strains. Ammonium lactate and acetamide showed significant effects upon the growth and differentiation of the eye-antennal discs in some mutant strains with the genetical backgrounds of the wild type, cultured together with their own cephalic complexes, and upon those of the eye-antennal discs of Oregon-R cultured together with the cephalic complexes of these mutant strains. Urea showed most marked effects upon the eye-antennal discs of several strains except Dp to promote the growth and differentiation of the wild type. Polypeptone had effects similar to urea on all strains except Dp. The eye-antennal discs of the Dp strain seem to be different from the other eye-mutant strains in the responsibility to the cephalic complexes of the wild type, although the cephalic complexes of this strain seem to be similar to other eye-mutant strains in promoting the growth and differentiation of the eye-antennal discs of the wild type in media containing acetamide.