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Studies on the factors affecting formation of the Fontes' granules, growth and other characteristics of Mycobacterium tuberculosis avium. IV. Studies on the fluctuation of virulence in Mycobacterium tuberculosis avium A62 strain induced by the serial transfer on the Ogawa media containing maleic hydrazide


Studies on the factors affecting formation of the Fontes' granules, growth and other characteristics of Mycobacterium tuberculosis avium. IV. Studies on the fluctuation of virulence in Mycobacterium tuberculosis avium A62 strain induced by the serial transfer on the Ogawa media containing maleic hydrazide



Shikoku Acta Med 10(3): 215-220



The author reports the change of virulence of M. tuberculosis avium during the course of serial transfer of the organism on the Ogawa media containing maleic hydrazide. Decrease in virulence was noted in the mutant obtained after repeated transfers on the media described above. Young cocks fed under the identical conditions since hatching of the eggs, were used to test virulence. The control group referred in this experiment is a group of cocks injected with the original virulent strain. Unless otherwise mentioned M-group signifies the group of cocks injected with the mutant obtained from the cultures containing maleic hydrazide. Though no decrease in body weight was recorded in either group before autopsy, the ratio of weight increase was lower in the control group. In 3 of the control groups, yellowish fat-like infiltration combined with granulous proliferation was observed at the locus of the injection of the bacilli. On the other hand, only one of the M-group showed the similar but milder yellowish infiltration at the injection site. Macroscopic tuberculous lesions in the internal organs: Liver: In the control group extremely large number of miliary tubercules was found in one animal and 2 showed a fairly large number of the lesions and 6 showed a small number of the tubercles, whereas in the M-group a fairly large number of tubercles was observed only in one test animal and 3 showed a small number of the miliar tubercles. The average weights of the infected livers of the both groups were 43.9 g for the control group and 25.7 g for the M-group. The lesion in the control was more severe. Spleen: Three in the control group showed a fairly large number of the miliary tubercles and other 3 manifested only a small number. In the M-group, however, only 3 showed a small of the tubercles. Average weight of the spleen was 8.9g for the control group and 5.3g for the M-group in which the lesion was more mild. Smear for the acid fast bacilli: Liver: In the control group, extremely large number of the bacilli was detected in one animal and fairly large number in one and 7 showed a small number of the bacilli. In the M-group fairly large number of the bacilli was detected in only 3. Spleen: A small number of the bacilli were observed in 6 of the control group and 3 in the M-group. It may be concluded from these data summarized above that the virulence of A62 strain of M. tuberculosis avium can be reduced by cultivating the organism on the Ogawa media containing 0.5% maleic hydrazide over the period of 9 months with 12 transfers.

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Studies on the factors affecting formation of the Fontes granules, growth and other characteristics of Mycobacterium tuberculosis avium III Morphological studies on the changes of A62 strain of Mycobacterium tuberculosis avium and its Fontes granules induced by the serial transfers on the Ogawa media containing maleic hydrazide. Shikoku Acta Med: 211-214, 1957

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