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Studies on the oxidative decomposition of urocanic acid by liver extract



Studies on the oxidative decomposition of urocanic acid by liver extract



I Jour Biochem [tokyo] 43(5): 603-609



A partially purified enzyme preparation, catalyzing oxidative decomposition of urocanic acid was obtained from aqueous extract of liver by ammonium sulfate fractionation between 0.3-0.5% saturation, Ca phosphate gel absorption and [image]/15 phosphate buffer (pH 8.4) elution. O2 uptake by this preparation with urocanic acid was increased greatly by the addition of ethylenediaminetetraacetate (EDTA). When the original aqueous extract was first treated with Norit for removal of impurities, the preparation is readily active on urocanic acid without EDTA. When a rabbit was given aminopterine (5 mg daily) for 40 days, the preparation from liver without Norit treatment was active on urocanic acid without addition of EDTA.

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