+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Studies on vascularization of the cornea. I Sensitization of the cornea of rabbits to bacteria. II. Sensitization of the cornea of rabbits to proteins. III. Sensitization of the cornea of monkeys. IV. The question of passive corneal hypersensitiveness



Studies on vascularization of the cornea. I Sensitization of the cornea of rabbits to bacteria. II. Sensitization of the cornea of rabbits to proteins. III. Sensitization of the cornea of monkeys. IV. The question of passive corneal hypersensitiveness



Jour Immunol 26(4): 267-280, 281-294, 295-302, 303-312



I. The exps. were predicated on the concept that pannus formation in trachoma may be in response to a specific bacterial hypersensitiveness. Several bacteria were studied in this connection, and observations made on the occurrence of corneal hypersensitiveness after repeated administration, by different routes, of heat-killed or living organisms. The data indicated that, of the bacteria utilized, Staphylococcus aureus possesses the most effective sensitizing antigen, and that the most consistent corneal reactivity is obtainable by direct sensitization of the scarified cornea. However, unless sufficient corneal trauma accompanies instillation of bacteria into the conjunctival sac, corneal hvpersensitivity apparently does not occur. It was shown, then, that the cornea of rabbits may be so sensitized as to develop marked clouding and vascularization. While it is not clear that the pannus of trachoma is related to corneal vascularization as here observed, in the beginning stage, these changes resemble so-called pannus tenuis of trachoma, and later are suggestive of pannus crassus. By repeated periodic scarification and instillation of bacteria, pannus may be maintained indefinitely. With cessation of instillations, the corneal changes undergo absorption, so that within wks. or mos. the vascularity is no longer visible under examination by hand-slit lamp. Using micropathoiogical methods, however, it was seen that the blood vessels never actually disappear.[long dash]II. It is shown that corneal clouding and vascularization may be stimulated entirely by a state of hypersensitiveness. Thus, the reactivity of the cornea of normal rabbits to proteins may be increased by repeated periodic inoculations of the protein, either intracutan. or intracorneally. Corneal reactivity follows most consistently the repeated intracorneal injections of protein. While the cornea receiving inoculations always acquires an exquisite reactivity, the cornea of the uninoculated eye may remain non-reactive. Skin reactivity to protein may be absent also, depending on the route of inoculation, so that no relationship is manifested between the sensitivity of either cornea, of the skin or the presence of circulating antibodies. Bacterial proteins (Staphylococcus and Bacterium granulosis) and native proteins (egg-albumin) are equally effective in inducing corneal reaction.[long dash]III. Response of the cornea of monkeys to repeated inoculations (scarification of cornea followed by instillation of conjunctival sac) of Staph. aureus was essentially a clouding and vascularization. Reactivity appeared to be local, since neither the cornea of the non-inoculated eye nor the skin acquired demonstrable increased activity; furthermore, no antibodies were detectable in the sera. Monkeys inoculated repeatedly in one eye with Bact. granulosis apparently did not acquire corneal reactivity, though they received 20 inoculations. This is comparable with previous results in rabbits. It is obvious, then, that conditions promoting corneal vascularization by Staph. aureus, are inadequate and ineffective for Bact. granulosis.[long dash]IV. A study was made, in rabbits, of the passive inducibility of the hypersensitive corneal reaction typified by clouding and vascularization, 3 methods being employed: (a) intravenous or local injection of specific precipitating serum followed later by injection of antigen (albumin), (b) combination of antiserum and antigen, (c) antigen and later antiserum[long dash]reversal of the method of passive anaphylaxis. By all 3 methods it was possible, in certain instances, to cause skin reactivity. Passive establishment of corneal reactivity was unsatisfactory. While the majority of the tests were negative, some were of the irritative variety, rather than typical of hypersensitive corneal reactions. It is not clear, therefore, whether the evidence indicates that corneal reactivity may be passively induced.

(PDF emailed within 1 workday: $29.90)

Accession: 025595733

Download citation: RISBibTeXText


Related references

Clinical and experimental studies on the problem of cornea transplantation. II. Studies on the transference of micromolecular compounds in the cornea and various kinds of corneal grafts in rabbits. Klinika Oczna 37(3): 315-321, 1967

Xenogeneic corneal grafting part 1 active and passive sensitization of the rat against guinea pig cornea. Albrecht Von Graefes Archiv fuer Klinische und Experimentelle Ophthalmologie 185(3): 235-244, 1972

Anomalous effects of dimethyl sulfoxide on the response of the mouse lens and cornea to x-irradiation: "protection" of lens and "sensitization" of cornea. Radiology 92(1): 156-158, 1969

Study of the effect of hydrophilic gel contact lenses on the cornea. I. Lactic acid in the cornea and aqueous humour and cornea hydration after application of contact lenses Geltakt in rabbits. Ophthalmologica. Journal International d'Ophtalmologie. International Journal of Ophthalmology. Zeitschrift für Augenheilkunde 159(4): 398-406, 1969

Studies with azlocillin, mezlocillin, penicillin-g-potassium and sisomicin on tolerance in the cornea and the kinetics of inhibiting concentrations in the cornea and aqueous humor in rabbits. Infection 10 Suppl 3: S221-S226, 1982

Studies on the cornea. III. The fine structure of the frog cornea and the uptake and transport of colloidal particles by the cornea in vivo. Journal of Cell Biology 15: 241-258, 1962

Studies on the cornea. I. The fine structure of the rabbit cornea and the uptake and transport of colloidal particles by the cornea in vivo. Journal of Cell Biology 12: 457-479, 1962

The innervation of the cornea. Research on the resting cornea in rabbits after mild scarification, intraocular infection with Box virus bovis and bilateral sympathectomy. Albrecht Von Graefe's Archiv für Ophthalmologie 162: 565-609, 1961

Effect of Donor Cornea on the Surgical Outcomes of Penetrating Keratoplasty: Imported Cornea Versus Domestic Cornea. Experimental and Clinical Transplantation 2018, 2018

Experimental study of the influence of cornea flap and residual cornea bed on cornea intensity after LASIK. Chinese Journal of Ophthalmology 39(3): 150-155, 2003

Circulating cornea-specific antibodies in corneal disease and cornea transplantation. Graefe'S Archive For Clinical & Experimental Ophthalmology. 232(2): 82-86, 1994

Transplantation of cryopreserved canine amniotic membrane for cicatrisation in cornea with limbal stem cells deficiency in rabbits FT Transplante de membrana amniotica canina criopreservada para cicatrizacao de cornea com deficiencia celulas limbicas em coelhos. 2007

Studies on the frozen stored cornea. III. Electron microscopic observation on the cornea. I. On the changes of the corneal epithelial cells after frozen storage. Nippon Ganka Gakkai Zasshi 65: 941-951, 1961

Healing of mechanical and thermal lesions of the corneal endothelium and posterior surface of the cornea in rabbits and rhesus monkeys. Klinische Monatsblaetter fuer Augenheilkunde 188(3): 225-230, 1986

Artificial scleral sinus with covering of recipient's cornea with cadaveric cornea for treating acute corneal staphyloma and keratoconus. Klinika Oczna 83(10-12): 535-536, 1981