+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Study of complex-forming processes in biopolymers by adsorption polarography

Study of complex-forming processes in biopolymers by adsorption polarography

Biofizika 9(5): 558-564

ISSN/ISBN: 0006-3029

Polarographic maxima were used to study the formation of complexes between DNA and proteins. Measurements were made on a series of tubes containing 1 ml crystalline proteinase from Bacillus subtilis (20 [mu]g/ml in 0.6 [image] NaCl) and 0.05-1 ml DNA from herring roe (50 [mu]g/ml) and from thyroid. Tubes were made up to 2 ml with 0.006 M NaCl after which 6 ml of maximum-forming reagents (Biokhimiya 26:575, 1961) were added. The process of complex formation in the absorption curves was represented by segments which were not a function of the concentration of one of the components in the complex (if the concentration of the other component is constant). Experiments with DNA depolymerization showed that in neutral medium and at pH 9 formation of a complex between DNA and subtilysin occurred in a 1:2 ratio. Highly polymerized thyroid DNA formed a complex in a 1:1400 ratio in a neutral medium (at complete saturation). Complexes of highly polymerized DNA and subtilysin are apparently formed by adsorption. It is possible that the inner surface of DNA's double helix also participates in complex formation. Polarographic maxima can be used to determine the degree of depolymerization of nucleic acids. Highly polymerized nucleic acids are able to suppress the maxima more than depolymerized DNA.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 025598610

Download citation: RISBibTeXText

Related references

Adsorption Polarography in the Study of Processes of Complex Formation of Bio-Polymers. Biofizika 9: 558-564, 1964

Pulsed NMR method for determining the thermodynamic characteristics of adsorption processes in biopolymers. Russian Journal of Physical Chemistry A, Focus on Chemistry 81(7): 1165-1169, 2007

Polarography of the alkaline-earth metals--II: The adsorption wave for the Magnesium-Eriochrome Black T complex. Talanta 32(6): 479-482, 1985

Adsorption of viruses on antigen-antibody complex; adsorption of mouse encephalomyelitis virus onto precipitate forming ex tempore. Voprosy Virusologii 3(1): 17-19, 1958

Analyses of drugs by polarography 23. determination by polarography differential pulse polarography and cathode ray polarography of triazolam in plasma and serum. Archiv der Pharmazie (Weinheim) 317(1): 70-73, 1984

Simultaneous Determination of Cadmium and Lead in Real and Environmental Samples by Differential Pulse Polarography after Adsorption of Their 2-Nitroso-1-naphthol-4-sulfonic acid Tetradecyldimethylbenzylammonium Ion-Associated Complex on Microcrystalline Naphthalene. Microchemical Journal 58(1): 21-30, 1998

Adsorption voltammetric techniques for the determination of uranium (VI) with 2,5-dichloro-3,6-dihydroxy-1,4-benzoquinone as complex forming reagent. Fresenius' Journal of Analytical Chemistry 349(8-9): 654-658, 1994

Processes forming the rock complex of a coal-bearing formation. Pages 86-94 1969, 1969

Classification and mapping of complex exogenic relief-forming processes. Geomorfologiya 1992(1): 25-29, 1992

The self-organisation concept; the forming of structures by complex dynamic processes. Congres Geologique International, Resumes 34: 3875, 2012

Ore-forming processes in the upper part of the Bushveld Complex, South Africa. Journal of African Earth Sciences 20(2): 77-89, 1995

Drug analyses by means of polarography or oscillo polarography part 18 the electro analytical properties of bromazepam and its determination in blood by means of cathode ray polarography. Archiv der Pharmazie (Weinheim) 308(9): 720-723, 1975

Adsorption of biopolymers. Colloids and Surfaces A: Physicochemical and Engineering Aspects 76: 159-166, 1993

Polarography with slowly forming mercury drops. Nature 163(4138): 290, 1949

Results of methodical investigations into the complex regulation of yield-forming processes in carnations. Acta Horticulturae 216: 331-337, 1987