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Study upon natural growth factor of L. leichmannii cultured in B. brevis



Study upon natural growth factor of L. leichmannii cultured in B. brevis



Rev Soc Argentina Biol 30(1/2): 27-32



The following results were obtained by simultaneous determination of "vitamin B12" in culture medium of B. brevis, containing peptone, using the chemical method of Boxer and Rickards and the microbiological method of Skeggs. The values obtained with Boxer and Rickards's method are too high as compared to those of the microbiological technique. A factor related to the peptone, without growth activity for L. leichmannii, is responsible for the high values found by the chemical method. If we accept that the growth factor for L. leichmannii produced by B. brevis corresponds to the vitamin B12 group, it would be a cobalamin different from the cyanocobalamin or vitamin B12.

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