Sulfated Insulin for Treatment of Insulin-Resistant Diabetics
Moloney, P.J.; Aprile, M.A.; Wilson, S.
Journal of New Drugs 4(5): 258-263
ISSN/ISBN: 0096-0284 PMID: 14218619 DOI: 10.1002/j.1552-4604.1964.tb00356.x
Sulfated insulins obtained by reaction of ox or pig insulin with H2SO4 had chemical and immunological properties very different from those of the parent insulin. The sulfated material was relatively non-neutralizable by antiserum against ox insulin, using the response of whole mice or mouse diaphragm tissue to indicate hormonal activity. It also reacted poorly with anti-ox-insulin serum in agar immuno-diffusion, complement fixation, and hemagglutination tests. Sulfated insulin was found to be much less antigenic in guinea pigs than unaltered insulin. Eleven insulin-resistant diabetic patients were brought under control with sulfated insulin at dosages which were always much lower, usually one-tenth to one-fifth, than those required when standard insulin preparations were employed. There was no tendency for the required dose of sulfated insulin to increase with prolonged therapy. As of June 1964, 5 cases were successfully treated for over 2 years. Two out of 5 patients who were allergic to ox and pig insulins were found to respond to sulfated insulin without unfavorable reactions. However, sulfated insulin showed no advantage over unaltered insulin for treatment of normal or unstable diabetics.