Sulfhydryl groups of liver hexokinase and glucokinase and their participation in catalysis of glucokinase reaction

Titova, G.V.

Vop Med Khim 12(5): 511-513


Accession: 025611547

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The complete bonding of sulhydryl groups by N-ethylmaleinimide and cystamine does not affect hexokinase activity. Hence, SH-groups of the enzyme do not take part in the catalysis of the hexokinase reaction. Decrease of activity of the preparation of specific liver glucokinase due to N-ethylmaleinimide and cystamine and the complete prevention of inhibition by cysteine shows that the presence of free sulfhydryl groups is necessary for the display of glucokinase activity.