The biosynthesis of adrenal steroids in vitro. L The conversion of endogenous and exogenous precursors in a pig adrenal cortex homogenate

Kahnt, F.W.; Neher, R.

Helv Chim Acta 48(7): 1457-1476

1965


Accession: 025692100

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Abstract
The conditions of the adrenal steroid biosynthesis in vitro were thouroughly studied, using beef adrenal cortex homogenate with endogenous and exogenous precursors. The preformed endogenous precursor was identified with cholesterol. Its aerobic incubation produced a 10-30-fold increase of the amount of originally present corticosteroids, such as cortisol, corticosterone, aldosterone, 18-hydroxycorticosterone and 19-hydroxy-corticosterone. From a wide variety of exogenous precursors studied so far, corticosterone was quantitatively the best precursor for aldosterone, whereas the pathway probably involves 18-hydroxy-corticosterone. The optimal conditions for the formation of aldosterone may be different from those for other corticosteroids. The methods used are fully described.