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The effect of DNP and NaN3 on fertilized eggs of the sea urchin with special reference to the induction of the abnormal cleavage



The effect of DNP and NaN3 on fertilized eggs of the sea urchin with special reference to the induction of the abnormal cleavage



Embryologia 5(1): 71-77



Fertilized eggs of the sea urchins, Pseudocentrotus depressus, Hemicentrotus pulcherrimus, Temnopleurus toreumaticus and Anthocidaris crassippina, were treated with DNP-sea water ([image]/500-[image]/4000) or with NaN3-sea water ([image]/100-[image]/ 400) for more than 30 minutes at various stages. When the treatment was carried out before the streak stage, the eggs divided into 2 cells after they were returned to sea water, but when the treatment was done later than the streak stage, the eggs divided directly into 4 cells. A similar procedure at the 2-cell or 4-cell stage induced division directly into 8 cells or 16 cells respectively. The DNA- or NaN3-treated eggs reached the 4-cell stage 20-30 minutes faster than the normal egg.

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Accession: 025738529

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DOI: 10.1111/j.1440-169x.1960.tb00266.x


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