The effect of environmental extremes on the detectibility of leukocyte isoantigens in normal humans with inference to tissue typing of prospective donors

Zmuewski, C.M.; Mccloskey, R.V.; Pierre, R.L.S.

Histocompatibility testing Report of a conference and workshop 14-24 June, Torino and Saint-Vincent, Italy 397-405


Accession: 025748605

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Certain seemingly normal environmental conditions can result in an artifactual change in the donor's leukocytic phenotype. Mild exercise, exposure to excessively hot or cold weather and overeating can cause a reduction in leukocyte reactivity and falsely negative reactions. Excessive exercise and the after-effects of overindulgence in alcohol create a state of hyperactivity and some falsely positive reactions. Consistent typing was possible only when the blood used for testing was collected early in the morning after adequate rest and before breakfast. Leukocyte reactivity can be decreased by a severe stress situation. The exact nature of the factors involved in increasing or decreasing reactivity are unknown. The decrease in reactivity observed following overeating may be due to some serum factors. Some heat labile factor in native serum that enhances agglutination is postulated.