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The effect of the inhibition of protein synthesis on the establishment of lysogeny

Virology 4(1): 53-71

The effect of the inhibition of protein synthesis on the establishment of lysogeny

The establishment of lysogeny in Escherichia coli strain C by phage P2 was studied. Treatment of infected cells with chloramphenicol, amino acid analogs, or 5-hydroxyuridine, or starvation for a required amino acid, increases the frequency of lysogenization, whereas treatment with cyanide, azide, dinitrophenol, or pretreatment with UV light has no effect. It was also found that treatment with chloramphenicol of cells of Salmonella typhimurium infected with phage P22 increases the frequency of lysogenization by this phage. It is therefore inferred that the selective inhibition of protein synthesis in infected cells tends to favor the lysogenic response over the lytic one. Treatment of infected cells with proflavine increases the frequency of lysogenization by P2. Chloramphenicol and proflavine are most effective in this respect when added about halfway through the latent period.

Accession: 025766149

PMID: 13456359

DOI: 10.1016/0042-6822(57)90043-0

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