EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The effect of the inhibition of protein synthesis on the establishment of lysogeny






Virology 4(1): 53-71

The effect of the inhibition of protein synthesis on the establishment of lysogeny

The establishment of lysogeny in Escherichia coli strain C by phage P2 was studied. Treatment of infected cells with chloramphenicol, amino acid analogs, or 5-hydroxyuridine, or starvation for a required amino acid, increases the frequency of lysogenization, whereas treatment with cyanide, azide, dinitrophenol, or pretreatment with UV light has no effect. It was also found that treatment with chloramphenicol of cells of Salmonella typhimurium infected with phage P22 increases the frequency of lysogenization by this phage. It is therefore inferred that the selective inhibition of protein synthesis in infected cells tends to favor the lysogenic response over the lytic one. Treatment of infected cells with proflavine increases the frequency of lysogenization by P2. Chloramphenicol and proflavine are most effective in this respect when added about halfway through the latent period.


Accession: 025766149

PMID: 13456359

DOI: 10.1016/0042-6822(57)90043-0



Related references

Cannon, R.E.; Shane, M.S., 1972: The effect of antibiotic stress on protein synthesis in the establishment of lysogeny of Plectonema boryanum. Virology 49(1): 130-133

Marin, G.; Basilico, C., 1967: Effect of the inhibition of protein synthesis on the establishment of transformation by polyoma virus. Puromycin was used to study the effect of the inhibition of protein synthesis on transformation of hamster cells (BHK21) by polyoma virus. The drug was used at a concentration (10-4 [image]) which caused in these cells a drastic but fully reversib...

Smith, H.O.; Levine, M., 1965: The Synthesis Of Phage And Host Dna In The Establishment Of Lysogeny. A method of extraction is described which prevents excessive fragmentation of bacterial DNA. The larger bacterial DNA can then be separated from the phage DNA on sucrose gradients. The relative amounts of phage and host DNA made at various times d...

Smith, H.O.; Levine, M., 1964: Two Sequential Repressions Of Dna Synthesis In The Establishment Of Lysogeny By Phage P22 And Its Mutants. The rate of DNA synthesis in complexes infected with wild type and mutants of phage P22 was followed by H3-thymidine pulse labeling. The essential features of the c+ infection, which gives high-frequency lysogeny, are (1) early onset of phage-spec...

Shearwin, K.E.; Egan, J.B., 2000: Establishment of lysogeny in bacteriophage 186. DNA binding and transcriptional activation by the CII protein. The CII protein of bacteriophage 186 is a transcriptional activator of the helix-turn helix family required for establishment of the lysogenic state. DNA binding by 186 CII is unusual in that the invertedly repeated half sites are separated by 20...

Anonymous, 1960: Lysogeny in the mycobacteria I The establishment of lysogeny

Russell, R.L.; Jann, G.J.; Froman, S., 1960: Lysogeny in the mycobacteria. 1. The establishment of lysogeny. American Review of Respiratory Disease 82: 384-393

Bertani, L.E., 1959: The effect of ultraviolet light on the establishment of lysogeny. The resistance to UV of cells of Shigella dysenteriae, infected with the temperate phage P2 or with various temperate and virulent mutants of P2, was measured at various times in the course of the latent period. The resistance of cells infected wi...

Manthey, J.; Pulverer, G., 1975: Lysogeny-typing of Staphylococcus aureus. Comparison of spontaneous lysogeny, UV ray-induced lysogeny and chemically induced lysogeny. Archivum Immunologiae et Therapiae Experimentalis 23(5): 631-638

Danis, C.; Mabrouk, T.; Garzon, S.; Lemay, G., 1993: Establishment of persistent reovirus infection in SC1 cells: Absence of protein synthesis inhibition and increased level of double-stranded RNA-activated protein kinase. In the present study we report the establishment and characterization of an SC1 cell line persistently infected by reovirus. We observed that a significant percentage of SC1 cells was resistant to cell lysis upon infection with non-defective reovi...