The Fine Structure of Stromalytic Forms Produced by Osmotic Hemolysis of Red Blood cells

Baker, R.F.

Journal of Ultrastructure Research 11: 494-507

1964


ISSN/ISBN: 0022-5320
PMID: 14244173
DOI: 10.1016/s0022-5320(64)80078-2
Accession: 025792419

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Abstract
When human red blood cells are hemolyzed by hypotonic salt solutions, many of the cells give rise to long cylindrical tubes which range from 200 A to 1 μ in diameter and may be several microns long. Negative staining of these preparations shows that the tubes are bounded by a membrane 40-60 A in width, are relatively impermeable to PTA, and show no periodic structures in either negative staining or in thin sections. Influenza virus (PR8) does not attach to the tubes. Reasons are given for supposing that the tubes are not strictly identical to myelin forms, but probably contain a high percentage of hemoglobin.