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The histochemical demonstration of histamine in mast cells

The histochemical demonstration of histamine in mast cells

Journal of Histochemistry and Cytochemistry 9: 534-541

The successful histochemical demonstration of mast cell histamine depended on adequate fixation. When rat tissues containing mast cells were frozen-dried and fixed in formaldehyde vapor, several histochemical reactions applied to these tissues indicated the presence of histamine in the mast cells. Diazonium coupling with diazotized p-bromoaniline, fluorescence and reducing properties were all consistent with the presence of intracellular histamine which has reacted with formaldehyde. Reactions performed on aqueous formaldehyde fixed tissues were weakly positive or negative.

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Accession: 025810434

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PMID: 14461582

DOI: 10.1177/9.5.534

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