+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The quantitative separation of purines, pyrimidines, and nucleosides by paper chromatography

The quantitative separation of purines, pyrimidines, and nucleosides by paper chromatography

Journal of Biological Chemistry 175(1): 315-332

By paper strip chromatography in a butyl alcohol system, cytosine, uracil, adenine, and thymine may be isolated from mixtures and from hydrolysates of nucleic acids; the migrating substances, extracted from segments of the paper, are identified and their quantity detd. by u.-v. spectrophotome-try. Guanine also can be recovered under favorable circumstances. In the same way the nucleosides cytidine, guanosine, adenosine, and thymidine are separable from each other and from most of the free bases. The composition of binary mixtures, if encountered, may be detd. quantitatively. The method outlined appears to provide a means of estimating with some accuracy the constituent purine and pyrimidine bases, and probably the nucleosides, in hydrolysates of nucleic acids. Chromatography of partial hydrolysates of nucleic acids reveals the rate of liberation of individual bases and nucleosides.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 025903662

Download citation: RISBibTeXText

PMID: 18873306

Related references

Separation of nucleotides, nucleosides, purines, pyrimidines and some pyrimidine degradation products by two-dimensional paper chromatography. Journal of Chromatography 20(3): 624, 1965

Paper chromatographic separation of nucleotides, nucleosides, purines, and pyrimidines. J Chromatogr 18(1): 81-85, 1965

A mildly alkaline (pH 8) solvent for the isolation, by paper chromatography, of purines, pyrimidines, and their nucleosides and nucleotides. Archives of Biochemistry and Biophysics 82(2): 485-487, 1959

Separation and determination of nucleosides, purines, pyrimidines and vitamins of the B group with bidimensional thin-layer chromatography. Bollettino Della Societa Italiana di Biologia Sperimentale 47(6): 163-165, 1971

Separation of nucleic acid hydrolysis products purines pyrimidines nucleosides nucleotides rna hydrolyzates and mixtures from nucleotide syntheses by column chromatography on amberlite xad 4. Journal of Chromatography 123(2): 347-354, 1976

Multilinear gradient elution optimization in reversed-phase liquid chromatography based on logarithmic retention models: application to separation of a set of purines, pyrimidines and nucleosides. Journal of Chromatography. a 1218(33): 5658-5663, 2011

Separation of nucleic acid hydrolysis products, purines, pyrimidines, nucleosides, nucleotides, ribonucleic acid hydrolyzates, and mixtures from nucleotide syntheses by column chromatography on amberlite XAD-4. Journal of Chromatography 123(2): 347-374, 1976

Research on nucleic acids. I. On the paper chromatographic determination of pyrimidines, purines and nucleosides. Hoppe-Seyler's Zeitschrift für Physiologische Chemie 322: 122-134, 1960

Liquid chromatographic separation of purines pyrimidines and their nucleosides on silica gel columns. Journal of Chromatography 211(3): 337-346, 1981

Chromatography of nucleotides, nucleosides, and pyrimidines and purines on activated charcoal. Jour Chromatog 3: 221-224, 1960

Separation of the purines and pyrimidines by ionophoresis on filter paper. Science 118(3075): 694-695, 1953

The paper chromatography of some purines, pyrimidines and imidazoles. Analyst 91(85): 526-528, 1966

Simultaneous determination of purines pyrimidines nucleosides and amino acids by single column chromatography. Science Tools 16(3): 39-42, 1969

2 dimensional inst thin layer high voltage electrophoresis and inst chromatography for the separation of urinary purines pyrimidines and pyrazolo pyrimidines. Clinica Chimica Acta: 319-330, 1969