Section 26
Chapter 25,906

The rate of post-radiation recovery of yeast cells on various media during the period preceding the mani-festations of radiation damage during cell division

Malinovskii, O.V.; Mityushova, N.M.

Doklady Akademii Nauk SSSR 170(2): 455-456


ISSN/ISBN: 0002-3264
Accession: 025905928

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An aqueous suspension of Saccharomyces cerevisiae (2 x 105 cells/ml) was gamma-irradiated at 800 rad/min and at a temperature of 4[degree]C. After irradiation, the culture was maintained at 30[degree] in water for 4 hours; at this point part of the culture was transferred to nutrient medium, while the rest remained in water for an additional 4 hours. After 8 hours the sur-vival rate was no different for the 2 cultures. Similar results were obtained with more dilute nutrient media. Recovery rates were similar under aerobic and anaerobic conditions, although sucrose (0.5% solution) was required for recovery under anaerobic conditions.

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