The rate of post-radiation recovery of yeast cells on various media during the period preceding the mani-festations of radiation damage during cell division
Malinovskii, O.V.; Mityushova, N.M.
Doklady Akademii Nauk SSSR 170(2): 455-456
1966
ISSN/ISBN: 0002-3264
Accession: 025905928
An aqueous suspension of Saccharomyces cerevisiae (2 x 105 cells/ml) was gamma-irradiated at 800 rad/min and at a temperature of 4[degree]C. After irradiation, the culture was maintained at 30[degree] in water for 4 hours; at this point part of the culture was transferred to nutrient medium, while the rest remained in water for an additional 4 hours. After 8 hours the sur-vival rate was no different for the 2 cultures. Similar results were obtained with more dilute nutrient media. Recovery rates were similar under aerobic and anaerobic conditions, although sucrose (0.5% solution) was required for recovery under anaerobic conditions.