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The use of dimethyl sulphoxide, glycerol. and reconstituted skim milk for the preservation of ram spermatozoa. L The tonicity and toxicity of dimethyl sulphoxide and reconstituted skim milk at 30 and 5 C



The use of dimethyl sulphoxide, glycerol. and reconstituted skim milk for the preservation of ram spermatozoa. L The tonicity and toxicity of dimethyl sulphoxide and reconstituted skim milk at 30 and 5 C



Australian J Biol Sci 18(4): 877-885



The viability of ram spermatozoa in milk and buffered saline diluents containing dimethyl sulphoxide and in milk diluents alone was investigated during incubation studies at 30 and 5[degree] C. Dimethyl sulphoxide did not contribute to the tonicity of a diluent. Dimethyl sulphoxide (5% v/v) or glycerol in a diluent depressed spermatozoal activity during incubation at 30 or 5[degree] C. Dimethyl sulphoxide retarded motility more than glycerol, especially with incubation at 5[degree] C, while glycerol rendered a greater proportion of cells immotile than did dimethyl sulphoxide, this effect being significant only after incubation at 30[degree] C. Reconstituted skim milk was better at 9% (w/v) than at 7 or 11% (w/v) at both incubation temperatures. Spermatozoa do not survive as well in a reconstituted skim milk diluent prepared for only 4 hr. as in a diluent prepared and stored at 5[degree] C for 1 or 2 days before use. Heating to 85[degree] C for 5 min. removed the toxic factor in pasteurized milk but had no effect on the reconstituted skim milk diluent.

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