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Utilization of a discontinuous buffer system in the starch-gel electrophoresis of hemoglobin



Utilization of a discontinuous buffer system in the starch-gel electrophoresis of hemoglobin



Rev Francaise Etud Clin Et Biol 3(8): 877-880



Starch-gel electrophoresis of normal and abnormal human hemoglobin in a discontinuous buffer system (tris-citrate and borate) instead of the usual continuous borate buffer system shows (a) a better resolution of the protein fractions with a clearer electrophoretic pattern; (b) in a solution of normal adult hemoglobin the presence of 2 minor components, A, and X, is clearly shown and also a fraction X2 not recognizable by other types of electrophoresis (paper, starch or starch-gel in continuous buffer system); (c) the presence of these same minor fractions in cord blood, although in smaller quantities.

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Accession: 026065447

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