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Vital coloring with nile blue and brilliant cresyl blue


Vital coloring with nile blue and brilliant cresyl blue



Protoplasma 48(4): 429-451



ISSN/ISBN: 0033-183X

The staining effect of the Oxazin dyes, Nile blue and Brilliant Cresyl blue, and compared. Cell sap is colored blue to green-blue by both. On the other hand the ionic coloring of clear cell saps is usually blue with Nile blue, violet with Brilliant Cresyl blue[long dash]exactly as the electro adsorptive coloring of cell walls. The fact that the customary brilliant Cresyl blue preparations contain significant amounts of Nile blue as an impurity, which was discovered by Drawert and Metzner, is reexamined (in high moor algae) as to the color expansion in pH graduated color series. Nile blue has a lower transition point. Correspondingly the expansion is made turbid by use of Brilliant Cresyl blue series, so that in the lowest pH stages the latter are affected by the Nile blue components where the stain penetrates. It is known that Nile blue stains polar constructed esters blue, neutral fats orange[long dash]red. It is shown upon continuation of necrosis in alga cells colored with Nile blue that the Nile blue is only admitted in the moment of the cell's death with an orange brown color of drops of reserve fat The color passage in these is therefore utilizable in cellular[long dash]physiological research as a death reaction. "Rapid staining" of clear cell saps may be forced if the cells are first submerged for a short time (1-2 min.) in a strong (neutral or acidic) dye bath of an electro[long dash]adsorptive membrane coloring and then are acted upon by a strong (n/15) alkaline phosphate buffer solution. The stain is then quickly taken up in the cell saps, which became vital blue to violet. If clear cell saps are destained through reduction of the color to leucoform, the vacuoule coloring will return almost instantly if 0.1% H2O2 is absorbed through the preparation.

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Accession: 026087310

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