Comparison of antigenic properties of ribosomal proteins from Novikoff hepatoma and normal liver
Wikman-Coffelt, J.; Howard, G.A.; Traut, R.R.
Biochimica et Biophysica Acta 277(3): 671-676
1972
ISSN/ISBN: 0006-3002
PMID: 4341782
DOI: 10.1016/0005-2787(72)90114-1
Accession: 026293915
Ribosomes from normal liver and Novikoff hepatoma ascites cells were compared by immunochemical techniques. Antisera were prepared against large and small subunits of each cell type. The reaction of these antisera with normal and hepatoma ribosomes, and the RNA and proteins derived therefrom, were compared. Two antigenic differences were observed between the proteins of the 60-S ribosomes from hepatoma cells and those from normal liver. The two proteins responsible for the immunological differences were isolated from the hepatoma 60-S ribosomal subunits by first preabsorbing tumor 60-S antiserum with normal 60-S ribosomal proteins and then reacting the supernatant with tumor 60-S proteins. The molecular weight of the two proteins thus isolated were 30 000 and 65 000 as determined by disc gel electrophoresis.