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Detection by immuno fluorescence of lipotropin producing cells in hypophysis in pigs



Detection by immuno fluorescence of lipotropin producing cells in hypophysis in pigs



General & Comparative Endocrinology 22(3): 365



Des immun-serums ont ere prepare a partir de lipotropine porcine (,B-LPH) apres couplage de l'hormone a de l'albumine bovine au rooyen de gluteraldehyde. Toutefois, l'immunserum brut ainsi obtenu ne montre pas une specificite exclusive vis-a-vis de la LPH et lorsqu'on le teste par hemo-agglutination, ii reagit egalernent avec l'ACTH (I-24) avec l'MSH et avec la,B-MSH. En immuno-fluorescence, cet immun-serum reagit avec les memes cellules hypophysaires que l'immun-serum anti-ACTH (1-24). 11 se localise a la fois sur des cellules a fines granulations acidophiles, sur des cellules uniquement positives au PAS, apres coloration au P AS-bleu alcian et sur les cellules du lobe intcrmediaire. Cependant, lorsqu'on purifie l'immun-serum anti-LPH en recourant a la chromatographie sur immuno-absorbant additionne d'ACTH (1-24), on obtient un produit qui, dans les tests d'hemo-agglutination ne donne plus de reaction qu'avec la LPH. En immuno-fluorescence, cet immun-serum purifie se localise uniquement sur Jes cellules PAS positives et sur le lobe intermediaire. Ces resultats suggerent que la lipotropine se comporte comme un precurseur de la,B-MSH et peut-etre meme de l'ACTH.

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