Free amino acids and amines in human dental plaque

Hyatt, A.T.; Hayes, M.L.

Archives of Oral Biology 20(3): 203-209

1975


ISSN/ISBN: 0003-9969
PMID: 1054979
DOI: 10.1016/0003-9969(75)90010-2
Accession: 026654658

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Abstract
Human, 24 hr dental plaque was extracted with either trichloroacetic acid or saline and the free amino acids and amines determined after partial purification by ion-exchange chromatography. Total amino acids, as represented by acid extractions, were measured by automated amino-acid analysis and gave a mean value (n = 6) of 12 μmoles/g wet wt of plaque, whereas the saline extracts contained a mean (n = 4) of 9 μmoles/g wet wt. The most prominent amino acids in the total extract were glutamate, 28 per cent, aspartate, 13 per cent, proline and glycine, each 12 per cent, and alanine 9 per cent. The main amino acids extracted with saline from the extracellular fluid were glutamate, 29 per cent, glycine, 18 per cent, alanine, 13 per cent, proline, 10 per cent and aspartate, 8 per cent. Acid-extractable amines were assayed fluorimetrically as their dansyl derivatives. They represented a mean (n varying between 7 and 28) of 2 μmoles/g wet wt comprising putrescine 71 per cent, cadaverine 11 per cent, and γ-aminobutyric acid 13 per cent. Histamine was also detected, 5 per cent, when assayed with o-phthalaldehyde. Agmatine and β-alanine were not detected in these extracts. The average concentrations of the amino acids and amines in the extracellular and intracellular pools were also estimated and the significance of these results has been discussed in terms of plaque amino-acid metabolism.