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Interrelationship of insulin and glucagon ratios on carbohydrate metabolism in isolated hepatocytes containing high glycogen



Interrelationship of insulin and glucagon ratios on carbohydrate metabolism in isolated hepatocytes containing high glycogen



Biochemical and Biophysical Research Communications 67(3): 1019-1027



The effect of physiological concentrations of glucagon and insulin on glycogenolysis was studied in the presence and absence of substrates in isolated hepatocytes containing high glycogen. In the absence of substrates glucagon stimulated glycogenolysis at 1014M concentration, and addition of 100 units of insulin partially inhibited glucagon stimulated glycogenolysis (1014M to 1011M). However, in the presence of substrates, insulin completely inhibited glucagon stimulated glycogenolysis (1014M to 1011M), indicating that molar glucagon and insulin ratios control carbohydrate metabolism in liver. Additional studies showed incorporation of amino acid into protein was linear for only 3 to 4 hr in cells containing low glycogen, whereas in cells containing high glycogen, incorporation was linear for 8 to 10 hr.

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Accession: 026838771

Download citation: RISBibTeXText

PMID: 1201057

DOI: 10.1016/0006-291x(75)90776-7


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