Investigation of decomposition of alkylating mutagens of the ethylenimine series in human lymphocyte culture
Bulletin Of Experimental Biology and Medicine: 689-691
ISSN/ISBN: 0007-4888 DOI: 10.1007/bf00797131
A simple method of determining the concentration of alkylating compounds in a culture medium with a high level of reproducibility is described. Thiophosphamide was shown not to decompose during 24 h either in the culture mixture or in a culture of human lymphocytes. Phosphemid, dipin, and fotrin underwent appreciable decomposition in culture in the course of 24 h, but these substances likewise were not decomposed during the first 6 h.