Perfusion of rabbit kidneys with cryoprotective agents

Pegg, D.E.

Cryobiology 9(5): 411-419


ISSN/ISBN: 0011-2240
PMID: 4568154
DOI: 10.1016/0011-2240(72)90158-7
Accession: 027126895

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Rabbit kidneys were perfused at 5 or 37 °C for 2 hr with 2 m solutions of ethylene glycol, glycerol, or dimethylsulfoxide. It was found that each cryoprotectant caused an initial decrease in vascular resistance which was greater at 5 than at 37 °C but that dimethylsulfoxide caused a subsequent increase in resistance which was due to endothelial damage. In each experiment at 5 °C there was an initial weight loss by the kidney followed by a steady weight gain not significantly different from that observed in control experiments. At 37 °C however, only glycerol produced an initial weight loss, and all three cryoprotectants, but especially glycerol, produced severe subsequent weight gain. It is suggested that this effect is due to the cryoprotectants increasing the permeability of capillary membranes. The implications of these findings for long-term organ storage are discussed, and it is argued that glycerol and ethylene glycol merit further study.