Ultrastructural demonstration of cystine in guinea pig back stratum corneum

Spearman, R.I.; Hardy, J.A.

Archiv für Dermatologische Forschung 251(4): 289-294

1975


ISSN/ISBN: 0003-9187
PMID: 47239
DOI: 10.1007/bf00562232
Accession: 027808588

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
A method for the ultrastructural demonstration of cystine in keratinized epidermal cells of guinea pig back hairy skin is described. This involves selective oxidation by peracetic acid and formation of an electron dense reaction product with uranyl acetate. Unkeratinized cells were unstained except for nuclei and keratohyalin. The thickened plasma membranes of transitional and keratinized cells were darkly stained. In transitional keratinized cells darkly stained membranes occurred initially in relation to desmosomes and then spread to intervening membrane areas. Dense material, probably matrix keratin containing cystine was found in the interiors of basal horny cells but not in the intermediate or superificial cells of the stratum corneum. It is suggested that the unstable matrix keratin is probably broken down by enzymatic hydrolysis above the basal zone of the stratum corneum.

Ultrastructural demonstration of cystine in guinea pig back stratum corneum