Home
  >  
Section 29
  >  
Chapter 28,016

Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements

Falkner, F.G.; Zachau, H.G.

Nature 310(5972): 71-74

1984

ISSN/ISBN: 0028-0836
PMID: 6330567
Accession: 028015964
Download citation:  
Text
  |  
BibTeX
  |  
RIS
Transcription of the immunoglobulin kappa light-chain genes depends on the presence of a TATA box upstream of the leader gene segment and is regulated by an enhancer sequence in the large intron. In studying a rearranged mouse kappa light-chain gene we have now found that sequences between--90 and--160 base pairs (bp) upstream of the coding region are essential for correct transcription in gene transfer experiments. This region contains the deca- and pentadecanucleotide sequences TNATTTGCAT and TGCAGCCTGTGNCCAG, which we call dc and pd, respectively. Sequences related to dc and pd were found upstream of all human and mouse kappa-chain variable region (Vk) genes, upstream of lambda-chain variable region (V lambda) genes, and within the mouse heavy-chain enhancer. An inverted and complementary form of the dc element (ATGCAAATNA, called cd) occurs upstream of all heavy-chain variable region (VH) genes. The newly defined sequences may be involved in the control of immunoglobulin gene transcription.

Full Text Article emailed within 1 workday: $29.90




Related References

Falkner, F.G.; Zachau, H.G. 1984: Correct transcription of an immunoglobulin κ gene requires an upstream fragment containing conserved sequence elements Nature (London) 310(5972): 71-74
Pelletier, M.R.; Hatada, E.N.; Scholz, G.; Scheidereit, C. 1997: Efficient transcription of an immunoglobulin kappa promoter requires specific sequence elements overlapping with and downstream of the transcriptional start site Nucleic Acids Research 25(20): 3995-4003
Sharp, S.J.; Garcia, A.D. 1988: Transcription of the Drosophila melanogaster 5S RNA gene requires an upstream promoter and four intragenic sequence elements Molecular and Cellular Biology 8(3): 1266-1274
Woudt, L.P.; Smit, A.B.; Mager, W.H.; Planta, R.J. 1986: Conserved sequence elements upstream of the gene encoding yeast ribosomal protein L25 are involved in transcription activation EMBO Journal 5(5): 1037-1040
Amemiya, K. 1989: Conserved sequence elements upstream and downstream from the transcription initiation site of the Caulobacter crescentus rRNAA gene cluster Journal of Molecular Biology 210: 5-54
Amemiya, K. 1989: Conserved sequence elements upstream and downstream from the transcription initiation site of the Caulobacter crescentus rrnA gene cluster Journal of Molecular Biology 210(2): 245-254
Amemiya, K. 1991: Conserved sequence elements upstream and downstream from the transcription initiation site of the Caulobacter crescentus rrnA gene cluster Journal of Molecular Biology 217(4): 751-752
Adzhalov, V.A.; Shakhmuradov, I.A. 1987: Nucleotide sequence of the gene of kappa immunoglobulin light chain produced by the hybridoma ptf 02 and some elements of transcription regulation Izvestiya Akademii Nauk Azerbaidzhanskoi SSR Seriya Biologicheskikh Nauk 5: 119-125
Clark, M.P.; Chow, C.W.; Rinaldo, J.E.; Chalkley, R. 1998: Correct usage of multiple transcription initiation sites and C/EBP-dependent transcription activation of the rat XDH/XO TATA-less promoter requires downstream elements located in the coding region of the gene Nucleic Acids Research 26(7): 1801-1806
Perry, M.; Thomsen, G.H.; Roeder, R.G. 1985: Genomic organization and nucleotide sequence of two distinct histone gene clusters from Xenopus laevis. Identification of novel conserved upstream sequence elements Journal of Molecular Biology 185(3): 479-499
Lötscher, E.; Siwka, W.; Zimmer, F.J.; Grummt, F.; Zachau, H.G. 1988: Transposed human immunoglobulin V kappa gene regions carry clusters of conserved sequence elements Gene 69(2): 225-236
Moreira, A.; Wollerton, M.; Monks, J.; Proudfoot, N.J. 1995: Upstream sequence elements enhance poly(A) site efficiency of the C2 complement gene and are phylogenetically conserved EMBO Journal 14(15): 3809-3819
De Souza, M.T.; Lecadet, M.M.; Lereclus, D. 1993: Full expression of the cryIIIA toxin gene of Bacillus thuringiensis requires a distant upstream DNA sequence affecting transcription Journal of Bacteriology 175(10): 2952-2960
Vankan, P.; Edoh, D.; Filipowicz, W. 1988: Structure and expression of the U5 snRNA gene of Arabidopsis thaliana. Conserved upstream sequence elements in plant U-RNA genes Nucleic Acids Research 16(22): 10425-10440
Emorine, L.; Kuehl, M.; Weir, L.; Leder, P.; Max, E.E. 1983: A conserved sequence in the immunoglobulin J kappa-C kappa intron: possible enhancer element Nature 304(5925): 447-449