+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Low antigen density tumor cells: an obstacle to effective autologous bone marrow purging?



Low antigen density tumor cells: an obstacle to effective autologous bone marrow purging?



Cancer Investigation 5(2): 113-118



Autologous bone marrow transplantation is a potentially curative approach to the treatment of various tumors that are refractory to conventional therapies. A major problem associated with the procedure is the possibility of tumor cell contamination in the autologous graft used to reconstitute the patient's immune system after supralethal chemoradiotherapy. A variety of different approaches to eliminating tumor cells from bone marrow have been proposed and tested. These include destruction of tumor cells with antibody and complement, use of antibody conjugated to drugs or toxins, and the physical separation of antibody-coated tumor cells by attachment to magnetic microspheres. Each of these approaches has different limitations and technical problems. One problem common to all, however, is that the tumor cells most likely to avoid removal are those demonstrating a low level of surface antigen. In this paper we have offered a practical approach to amplifying the unique surface antigen expression in order to enable this elusive tumor cell population to be eliminated. The approach proposed is adaptable to all the techniques currently being studied, since it is designed to add additional antigens to tumor cells which can then be used as targets at which to direct the various purging strategies.

(PDF emailed within 0-6 h: $19.90)

Accession: 028650958

Download citation: RISBibTeXText

PMID: 3300891

DOI: 10.3109/07357908709018465


Related references

Purging in autologous bone marrow transplantation importance of antigen density on leukemia blasts for the efficiency of complement mediated cytolysis. Leukemia Research 10(1): 114, 1986

Purging in autologous bone marrow transplantation relevance of target antigen density on blasts to the efficiency of complement mediated lysis. Bone Marrow Transplantation 1(SUPPL 1): 280, 1986

Immunologic purging in autologous bone marrow transplantation relevance of target antigen density on blasts to the efficiency of complement mediated cytolysis. Journal of Cellular Biochemistry Supplement (10 PART D): 217, 1986

Long-term culture of autologous bone marrow for marrow reconstitution following high-dose chemotherapy. Purging with tumor surface antigen directed human-human monoclonal antibody. Progress in Clinical and Biological Research 389: 111-118, 1994

The role and methodology for purging tumor from autologous bone marrow and peripheral blood progenitor cells. Medical Oncology 11(2): 47-51, 1994

The role of methodology for purging tumor from autologous bone marrow and peripheral blood progenitor cells. Medical Oncology (Northwood) 11(2): 47-51, 1994

Purging bone marrow for autologous transplantation are methods for monitoring the elimination of tumor cells reliable?. Experimental Hematology (Charlottesville) 11(SUPPL 14): 7, 1983

Purging of tumor contaminated human bone marrow utilizing autologous lymphokine activated killer cells. Clinical Research 35(3): 523A, 1987

High efficiency gene transfer to tumor cells Implications for genetic purging of autologous bone marrow. Blood 88(10 SUPPL 1 PART 1-2): 290B, 1996

Bone marrow resident memory T cells are not affected by both previous chemotherapy and marrow purging in patients given autologous bone marrow transplantation. Blood 96(11 Part 1): 789a, November 16, 2000

Triarylmethane dyes as therapeutic agents for the photochemical purging of autologous bone marrow grafts from residual tumor cells. Biophysical Journal 78(1 Part 2): 256A, 2000

Future paradigm for autologous bone marrow transplantation: tumor purging and ex vivo production of normal stem and progenitor cells. Journal of HematoTherapy 3(3): 213-218, 1994

Elimination of clonogenic tumor cells from HL-60, Daudi, and U-937 cell lines by laser photoradiation therapy: implications for autologous bone marrow purging. Blood 73(4): 1059-1065, 1989

Laser light induced photosensitization of leukemic cells and bone marrow cells implication for autologous bone marrow purging. Calcified Tissue International 41(SUPPL 2): 70, 1987

In vitro purging of clonogenic leukemic cells from human bone marrow by heat: Simulation experiments for autologous bone marrow transplantation. Leukemia Research 16(10): 973-977, 1992