Respiratory electron transport in plasma and thylakoid membrane preparations from the cyanobacterium anacystis nidulans

Veronique Molitor; Günter A.P.schek

FEBS Letters 195(1-2): 0-150

1986


ISSN/ISBN: 0014-5793
DOI: 10.1016/0014-5793(86)80149-1
Accession: 029087411

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Abstract
Plasma and thylakoid membranes were separated and purified from cell-free extracts of Anacystis nidulans by discontinuous sucrose density gradient centrifugation. Plasma membranes contained less than 0.05% (w/w) chlorophyll per protein. Both plasma and thylakoid membranes oxidized horse heart ferrocytochrome c (10 ± 4 and 3 ± 1 per mg protein, respectively), with strong inhibition by low concentrations of cyanide, sulfide, azide, carbon monoxide, and salicyl aldoxime. The activity was stimulated 125% by 3.5% Tween 80 and totally suppressed by 100 m M Na Cl. Oxidized cytochrome c was reduced by both types of membranes in the presence of Nad (P)H. The results indicate the occurrence of a respiratory chain including cytochrome-c oxidase in both plasma and thylakoid membranes of A. nidulans.