+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

A biochemical investigation into the function of corpus luteum A study of blood sugar and non-protein nitrogen changes in rabbits after the administration of corpus luteum

A biochemical investigation into the function of corpus luteum A study of blood sugar and non-protein nitrogen changes in rabbits after the administration of corpus luteum

Amer Jour Physiol: 586-596

Intraperitoneal injections of the Corner and Allen lipid extract of corpus luteum were given to rabbits (normal non-pregnant, pregnant, lactating and spayed does, and normal bucks) and resulted at certain times in marked physical reactions accompanied by rises in the sugar and non-protein N of the blood. Control experiments demonstrated that these reactions were specific for the active corpus luteum principle of the extracts used. In most cases, non-pregnant doe rabbits, pregnant rabbits after the 4th day of gestation and lactating rabbits after the 6th day postpartum gave no physical or biochemical evidence of responding to the injections. Occasionally, however, marked typical reactions occurred. No correlation could be made between such reactions and the known physiological state of the animals. Normal doe rabbits always react typically to injection any time from 34 hrs. to 4 days after copulation. The ovaries at this time show ruptured follicles. If the animal is spayed after being served the reaction does not follow injection. From 24 hrs. to 6 days after delivery normal does give positive reactions to the injection of corpus luteum. The post-delivery ovaries show no evidence of ruptured follicles and removal of them does not prevent the reaction. Removal of the uterus has no effect upon the response to corpus luteum either in early pregnancy or after delivery. Spayed does and normal bucks rarely show any physical or biochemical changes following intraperitoneal injection of corpus luteum, but if these unreactive animals are first brought under the influence of folliculin, the corpus luteum injection always induces a positive reaction. This finding indicates that the consistent reactions of does after copulation and the occasional reactions of non-pregnant and pregnant does are the result of the normal production of folliculin within the organism. This biochemical evidence confirms the findings of Hisaw and of Allen of an interrelationship between corpus luteum and the oestrus hormone. The behavior of lactating does, however, cannot be explained upon this basis and the possible influence of the sex principle of the anterior pituitary has not been eliminated. The rise in non-protein N is almost entirely accounted for by a rise of urea. Although this points to renal upset, the direct poisoning of the kidneys by lithium urate does not give comparable sugar and urea curves. Intraperitoneal injection of epinephrine, on the other hand, results in both non-protein N and sugar rises practically identical, in magnitude and time relations, to those following a positive corpus luteum reaction. This suggests that the corpus luteum under certain physiological conditions either stimulates the sympathetic nervous system or else has a direct effect upon the organism similar to that of the adrenal medulla. Whether or not the urea rise is a secondary result and possibly due to kidney damage has not yet been ascertained. It seems possible that the biochemical changes following injection of single large doses of corpus luteum extract may be an exaggeration of a normal catabolic action of this gland. This effect would be entirely in keeping with the importance of the corpus luteum for the implantation and nourishment of the ovum.

(PDF emailed within 1 workday: $29.90)

Accession: 029597488

Download citation: RISBibTeXText

Related references

Effect of hCG administration during corpus luteum establishment on subsequent corpus luteum development and circulating progesterone concentrations in beef heifers. Reproduction, Fertility, and Development 26(3): 367-374, 2015

Persistent versus transient stimulation of the macaque corpus luteum during prolonged exposure to human chorionic gonadotropin: a function of age of the corpus luteum. Endocrinology 114(6): 2175-2182, 1984

Physiological and clinical aspects of corpus luteum function and embryo-maternal interrelationships in ruminants, pigs and horses in the pre-implantation phase of pregnancy. I. Function of corpus luteum. Review article. Magyar Allatorvosok Lapja 49(5): 261-264, 1994

The corpus luteum reactivation test a simple method characterizing corpus luteum function. Acta Endocrinologica Supplementum 212: 124, 1977

Function of a corpus luteum formed before the ovulation of a persistent dominant follicle, in Holstein cattle treated with an intravaginal controlled internal drug-releasing device, in the absence of a corpus luteum. Veterinaria Mexico 30(1): 95-98, 1999

Corpus luteum therapy Function of the corpus luteum; progestin; relaxin; availability of progestin; clinical possibilities; empirical preparations. Jour Amer Med Assoc1: 1899-1901, 1935

Artificial prolongation of the life of the corpus luteum of pregnancy of the rabbit. Behaviour of the uterine horn and mammary gland of rabbits when the life of the corpus luteum of pregnancy is artificially prolonged. C. R. Soc. Biol, Paris, 130: 929-933, 1939

The corpus luteum reactivation test (CLR-test): a simple method for the characterization of the corpus luteum function (proceedings). Archiv für Gynakologie 224(1-4): 424-425, 1977

Physiology of the corpus luteum. V. The preparation and some chemical properties of progestin, a hormone of the corpus luteum which produces progestational proliferation. Amer Jour Physiol 91(1): 174-188, 1930

Expression of melatonin and its related synthase and membrane receptors in the oestrous corpus luteum and corpus luteum verum of sheep. Reproduction in Domestic Animals 53(5): 1142-1148, 2018

Mechanisms for rescue of corpus luteum during pregnancy: gene expression in bovine corpus luteum following intrauterine pulses of prostaglandins E1 and F2α. Biology of Reproduction 98(4): 465-479, 2018

The anovular corpus luteum in the golden hamster [Mesocricetus auratus Waterhouse) and comparisons with the normal corpus luteum and follicle of atresia. Acta Anatomica 66(2): 199-225, 1967

Volume of luteal tissue and concentration of serum progesterone in cows bearing homogeneous corpus luteum or corpus luteum with cavity. Animal Reproduction Science 49(2-3): 77-82, 1998

Variations in ovulatory response associated with the use of prostaglandins to manipulate the lifespan of the normal diestrous corpus luteum or the prolonged corpus luteum of the mare. Proceedings of the annual convention American Association of Equine Practitioners: ub 1979) (24th) 173-175, 1979

Effects of HCG stimulation in cattle with a cystic or compact corpus luteum: progesterone profile, ovulation induction and information of a second corpus luteum. Tierarztliche Umschau 43(7): 430...439, 1988