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A serological study of cell walls of certain lactic acid bacteria



A serological study of cell walls of certain lactic acid bacteria



VIIIth International Congress for Microbiology, Abstracts, Montreal, Quebec, Canada p 127



From three species of Lactobacillus and three species of Streptococcus cultured in a synthetic medium, cell walls were isolated (following sonic disintegration) and purified by washing. Sera against each species were prepared by injecting three rabbits with cell walls and three with intact cells. Reciprocal agglutination tests were carried out with unabsorbed and absorbed antisera. More kinds of antibodies were detected with cell-wall antisera than with intact-cell antisera. Many species in the two genera shared common antigens (S. faecalis was the exception). Certain antigens believed to be complex haptens in nature reacted with heterologous antisera. Haemagglutination of tanned erythrocytes sensitized with a participate cell-wall suspension showed fewer cross reactions than agglutination of intact-cell suspensions. The evidence presented shows the possibility of using antisera against species-specific cell-wall antigens for the identification of these species. The relationship of these species is discussed.

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Accession: 029784523

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DOI: 10.1139/m62-084


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