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A study of coliform organisms in the Melbourne water supply and in animal faeces, with observations on their longevity in faeces and in soil



A study of coliform organisms in the Melbourne water supply and in animal faeces, with observations on their longevity in faeces and in soil



Journal of Hygiene 46(3): 269-279



Bacteriological examination of 1616 samples of Melbourne water gave a higher percentage of confirmed tests for coliforms with MacConkey broth than with lactose broth. In studies of water and feces, confirmation by means of MacConkey broth incubated at 44[degree]C plus Koser citrate broth incubated at 37[degree]C was more specific for Escherichia coli I, gave a higher proportion of E. coli I, and showed the presence of greater numbers of intermediate-aerogenes-cloacae organisms, than subculturing on plates incubated at 37[degree]C. The types of organisms isolated by various methods from various sources were recorded. Coliform counts on decaying vegetation, and on algae from screens and sumps, were high, and numbers increased during incubation. Cow dung showed a gradual decrease in coliform content, corresponding to a decrease in moisture content. Heavily manured soil remained heavily contaminated for 3 years. When soils which were poor in organic matter were watered with cultures of coliforms, the organisms tended to die out.

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Accession: 029798924

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PMID: 18122186

DOI: 10.1017/s002217240003641x


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